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Robust heat shock induces eIF2α-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae

SYSNO ASEP	0326270
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Robust heat shock induces eIF2α-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae
Author(s)	Groušl, Tomáš (MBU-M)_{RID, ORCID} Ivanov, P. (CZ) Frýdlová, Ivana (MBU-M) Vašicová, Pavla (MBU-M)_RID Janda, Filip (MBU-M) Vojtová, Jana (MBU-M)_{RID, ORCID} Malínská, Kateřina (MBU-M) Malcová-Janatová, Ivana (MBU-M)_RID Nováková, Lenka (MBU-M) Janošková, Dana (MBU-M) Valášek, Leoš (MBU-M)_RID Hašek, Jiří (MBU-M)_{RID, ORCID}
Source Title	Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533 Roč. 122, č. 12 (2009), s. 2078-2088
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	p-bodies ; stress granules ; yeast
Subject RIV	EE - Microbiology, Virology
R&D Projects	GA204/02/1424 GA ČR - Czech Science Foundation (CSF)
	GA204/05/0838 GA ČR - Czech Science Foundation (CSF)
	GA204/09/1924 GA ČR - Czech Science Foundation (CSF)
	LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ME 939 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000266634800016
DOI	10.1242/jcs.045104
Annotation	Formation of stres granules (SGs) has not yet been described in S. cerevisiae. We showed that robust heat shock induced accumulation of eIF3a/Tif32p/Rpg1p in cytoplasmic foci. They also contained eIF3b/Prt1p, eIF3c/Nip1p, eIF4G2, Pab1p, mRNA and small but not large ribosomes and eIF2a/Sui2p. Surprisingly, Dcp2p and Dhh1p P-body markers also co-localized with eIF3a, however, since formation of eIF3-accumulations required different scaffolding proteins than P-bodies, we suggest that they represent yeast specific SGs
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2009

Number of the records: 1