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Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling
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SYSNO ASEP 0326242 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling Title Detekce extracelulární fosfatázové aktivity na úrovni jednotlivých buněk metodou Enzyme-Labeled Fluorescence a průtokovou cytometrií Author(s) Duhamel, S. (FR)
Gregori, G. (FR)
Van Wambeke, F. (FR)
Nedoma, Jiří (BC-A) RID, ORCIDSource Title Cytometry. Part A. - : Wiley - ISSN 1552-4922
75A, č. 2 (2009), s. 163-168Number of pages 6 s. Language eng - English Country US - United States Keywords alkaline phosphatase ; ELF phosphate ; heterotrophic bacteria Subject RIV DA - Hydrology ; Limnology CEZ AV0Z60170517 - HBU-Z, BC-A (2005-2011) UT WOS 000262921900011 DOI 10.1002/cyto.a.20686 Annotation ELF97 phosphate (ELF-P) is a useful compound for assessing the phosphorus-related status of planktonic aquatic populations. We have used a recently developed protocol that enables the detection by flow cytometry of ELF alcohol (ELFA), the product of ELF-P hydrolysis. The percentage of ELFA-labeled cells, the relative median ELFA fluorescence per cell, and the absolute ELFA fluorescence were determined in both freshwater (lake) and marine samples. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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