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Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system
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SYSNO ASEP 0326146 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system Title Vývoj funkčních LH receptorů u prasečích oocyt-kumulárních komplexů kultivovaných in vitro novým modelem dvoustupňové kultivace Author(s) Procházka, Radek (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Nagyová, Eva (UZFG-Y) RID, ORCID
Scsuková, S. (SK)
Mlynarčíková, A. (SK)Source Title Molecular Reproduction and Development - ISSN 1040-452X
Roč. 76, č. 8 (2009), s. 751-761Number of pages 11 s. Language eng - English Country US - United States Keywords LH Receptors ; oocyte Subject RIV ED - Physiology R&D Projects GA523/08/0111 GA ČR - Czech Science Foundation (CSF) QG50052 GA MZe - Ministry of Agriculture (MZe) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000268006400006 DOI 10.1002/mrd.21039 Annotation We show in the present study that freshly isolated pig cumulus-oocyte complexes (COCs) display a limited response to LH, as assessed by the expression of hyaluronan synthase 2 (Has2) mRNA, activation of protein kinase A (PKA), production of hyaluronic acid (HA) and progesterone, cumulus cell expansion and resumption of meiosis. These data indicate that freshly isolated COCs do not possess a sufficient number of functional LH receptors (LHR). However, the expression of Lhr significantly increased during the culture of COCs in vitro in a medium supplemented with FSH. Assuming that the effect of FSH on LHR induction is mediated via cAMP signaling pathways, we developed a new culture system, in which the COCs were pre-cultured for 72 h in a medium supplemented with dbcAMP. The pre-cultured COCs remained in the germinal vesicle stage, their cumulus investment underwent a dramatic increase in size and gap junctions between the cumulus cells were preserved. The stimulation of such COCs with either FSH or LH led to the resumption and completion of meiosis, activation of PKA, expression of Has2, synthesis of large amounts of HA and progesterone, and extensive expansion of cumulus cells. We conclude that the formation of functional LHR is stimulated in cumulus cells during the culture in vitro in a cAMP-dependent pathway. The dbcAMP-treated COCs thus represent a new model in which the resumption of meiosis and cumulus expansion can be induced exclusively by the action of recombinant LH. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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