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Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera
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SYSNO ASEP 0326027 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera Title Srovnávací studie metod SPR a ELISA založená na analýze vzorků krevního séra pacientů s rakovinou a zdravých jedinců na přítomnost CD166/ALCAM Author(s) Vaisocherová, Hana (URE-Y) RID
Faca, V.M. (US)
Taylor, A. D. (US)
Hanash, S. (US)
Jiang, S. (US)Source Title Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 24, č. 7 (2009), s. 2143-2148Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords SPR ; ELISA ; Cancer biomarkers Subject RIV CE - Biochemistry CEZ AV0Z20670512 - URE-Y (2005-2011) Annotation In this work, an ptimized SPR assay was implemented and its performance compared with an ELISA assay for CD166/activated cell leukocyte adhesion molecule (ALCAM), as candidate pancreatic cancer marker, based on direct and amplified detection in buffer and in human serum samples from healthy individuals and subjects with cancer. It is demonstrated that SPR can distinguish between ALCAM levels in cancer and control sera using direct detection without the need for additional amplification steps. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2009
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