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Separation of PCR-ready DNA from dairy products using magnetic hydrophilic microspheres and poly(ethylene glycol)-NaCl water solutions

SYSNO ASEP	0324026
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Separation of PCR-ready DNA from dairy products using magnetic hydrophilic microspheres and poly(ethylene glycol)-NaCl water solutions
Title	Separace DNA pro PCR z mléčných produktů pomocí magnetických hydrofilních mikročástic a polyethylenglykol-NaCl vodných roztoků
Author(s)	Rittich, B. (CZ) Španová, A. (CZ) Šálek, P. (CZ) Němcová, P. (CZ) Trachtová, Š. (CZ) Horák, Daniel (UMCH-V)_{RID, ORCID}
Source Title	Journal of Magnetism and Magnetic Materials. - : Elsevier - ISSN 0304-8853 Roč. 321, č. 10 (2009), s. 1667-1670
Number of pages	4 s.
Action	International Conference on Scientific and Clinical Applications of Magnetic Carriers /7./
Event date	20.05.2008-24.05.2008
VEvent location	Vancouver
Country	CA - Canada
Event type	WRD
Language	eng - English
Country	NL - Netherlands
Keywords	magnetic microsphere ; P(HEMA-co-GMA) ; DNA isolation
Subject RIV	CD - Macromolecular Chemistry
R&D Projects	GA203/09/1242 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
UT WOS	000265278000076
DOI	10.1016/j.jmmm.2009.02.110
Annotation	Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) and magnetic glass microspheres were used for the isolation of bacterial DNA. P(HEMA-co-GMA) microspheres were prepared by the dispersion polymerization in toluene/2-methylpropan-1-ol mixture in the presence of magnetite nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) salts with ammonium hydroxide. Carboxyl groups were then introduced by oxidation of the microspheres with potassium permanganate.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2009

Number of the records: 1