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Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)

SYSNO ASEP	0324020
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)
Title	Absence exprese spermatozoálního CD46 proteinu je spojena s rychlou akrozomální reakcí u myšice temnopásé (Apodemus agrarius)
Author(s)	Clift, L. E. (GB) Andrlíková, P. (CZ) Frolíková, M. (CZ) Stopka, P. (CZ) Bryja, Josef (UBO-W)_{RID, SAI, ORCID} Flanagan, B. F. (GB) Johnson, P. M. (GB) Dvořáková-Hortová, K. (CZ)
Source Title	Reproductive Biology and Endocrinology. - : BioMed Central Roč. 7, č. 29 (2009), s. 1-9
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	sperm competition ; acrosome reaction ; mating system
Subject RIV	EG - Zoology
CEZ	AV0Z60930519 - UBO-W (2005-2011)
UT WOS	000266491100001
EID SCOPUS	65549159123
DOI	10.1186/1477-7827-7-29
Annotation	In rodents, the cell surface complement regulatory protein CD46 is expressed solely on the spermatozoal acrosome membrane. Ablation of the CD46 gene is associated with a faster acrosome reaction. Sperm from three Apodemus species fail to express CD46 protein and exhibit a more rapid acrosome reaction rate than house mice (Mus). Here, we demonstrate that highly promiscuous species A. agrarius transcribe two unique alternatively spliced testicular CD46 mRNA transcripts, which differ from those described previously in other Apodemus species. In addition, A. agrarius CD46 transcripts have an extended AU-rich 3'-untranslated region (UTR) and a truncated 5'-UTR, resulting in failure to express spermatozoal CD46 protein. We show that A. agrarius has a significantly faster spontaneous acrosome reaction rate than A. sylvaticus and Mus. This provides a strategy to increase competitive sperm advantage for individuals, leading to faster fertilisation in this highly promiscuous genus.
Workplace	Institute of Vertebrate Biology
Contact	Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
Year of Publishing	2009

Number of the records: 1