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Laccase immobilized on magnetic carriers for biotechnology applications
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SYSNO ASEP 0323931 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Laccase immobilized on magnetic carriers for biotechnology applications Title Magnetické nosiče s imobilizovanou lakázou pro biotechnologické aplikace Author(s) Rotková, J. (CZ)
Šuláková, R. (CZ)
Korecká, L. (CZ)
Zdražilová, P. (CZ)
Jandová, M. (CZ)
Lenfeld, Jiří (UMCH-V) RID
Horák, Daniel (UMCH-V) RID, ORCID
Bílková, Z. (CZ)Source Title Journal of Magnetism and Magnetic Materials. - : Elsevier - ISSN 0304-8853
Roč. 321, č. 10 (2009), s. 1335-1340Number of pages 6 s. Action International Conference on Scientific and Clinical Applications of Magnetic Carriers /7./ Event date 20.05.2008-24.05.2008 VEvent location Vancouver Country CA - Canada Event type WRD Language eng - English Country NL - Netherlands Keywords macroporous bead cellulose ; laccase ; oriented immobilization Subject RIV CD - Macromolecular Chemistry R&D Projects GA203/09/0857 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000265278000002 DOI 10.1016/j.jmmm.2009.02.034 Annotation Laccase catalyzing the oxidation of p-diphenols has been applied in many industrial and biotechnology areas. Immobilized form of laccase has over come the problem with contamination of the final product. Nevertheless sensitive enzymes immobilized to the matrix can be inactivated by the environmental conditions. The aim of this research was to prepare carrier with improved activity and responsible stability even under extreme reaction conditions. Laccase immobilized through carbohydrate moieties on magnetic hydrazide bead cellulose with a final activity of 0.63 I.U./1ml of settled carrier confirmed that carriers with oriented immobilized enzyme might be useful in routine biocatalytic applications. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2009
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