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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
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SYSNO ASEP 0323396 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments Title MIQE: Minimum informací pro publikování experimentů s použitím kvantitativního real time PCR Author(s) Bustin, S.A. (GB)
Benes, V. (DE)
Garson, J.A. (GB)
Hellemans, J. (BE)
Huggett, J. (GB)
Kubista, Mikael (BTO-N) RID
Mueller, R. (US)
Nolan, T. (US)
Pfaffl, M.V. (DE)
Shipley, G.L. (US)
Vandesompele, J. (US)
Wittver, C.T. (US)Source Title Clinical Chemistry. - : Oxford University Press - ISSN 0009-9147
Roč. 55, č. 4 (2009), s. 611-622Number of pages 12 s. Language eng - English Country US - United States Keywords qPCR ; MIQE ; publication of experiments data Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA500520809 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000264774200006 Annotation Currently, a lack of consensus exists on how best to perform and interpret quantitative realtime PCR (qPCR) experiments. The problem is by a lack of sufficient experimental detail in many publications.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2009
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