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Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specificity and kinetics

SYSNO ASEP	0323280
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specificity and kinetics
Title	Proteiny SMH z Arabidopsis thaliana: kvantitativní studie telomer-vazebné specifity a kinetiky
Author(s)	Hofr, C. (CZ) Šultesová, P. (CZ) Zimmermann, M. (CZ) Mozgová, I. (CZ) Procházková Schrumpfová, P. (CZ) Wimmerová, M. (CZ) Fajkus, Jiří (BFU-R)_{RID, ORCID}
Source Title	Biochemical Journal. - : Portland Press - ISSN 0264-6021 Roč. 419, - (2009), s. 221-228
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	fluorescence anisotrophy ; single-Myb-histone protein (SMH protein) ; surface plasmon resonance
Subject RIV	BO - Biophysics
R&D Projects	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	IAA500040801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50040507 - BFU-R (2005-2011)
	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000264642800024
DOI	10.1042/BJ20082195
Annotation	We performed the first quantitative DNA-binding study of the plant-specific family of SMH proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics. Based on these data, a model explaining the binding stoichiometry and the protein arrangement on telomeric DNA is presented.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2009

Number of the records: 1