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Analysis of nrDNA polymorphism in closely related diploid sexual, tetraploid sexual and polyploid agamospermous species
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SYSNO ASEP 0323148 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of nrDNA polymorphism in closely related diploid sexual, tetraploid sexual and polyploid agamospermous species Title Analýza nrDNA polymorfismu u blízce příbuzných diploidních sexuálních, tetraploiních sexuálních a polyploidních agamospermických druhů Author(s) Záveská Drábková, Lenka (BU-J) RID
Kirschner, Jan (BU-J) RID, ORCID, SAI
Štěpánek, Jan (BU-J) RID, ORCID
Záveský, L. (CZ)
Vlček, Čestmír (UMG-J) RIDNumber of authors 5 Source Title Plant Systematics and Evolution. - : Springer - ISSN 0378-2697
Roč. 278, 1-2 (2009), 85, app.1-11Number of pages 19 s. Language eng - English Country AT - Austria Keywords Taraxacum ; ITS1-5.8S-ITS2 ; reticulate evolution Subject RIV EF - Botanics R&D Projects GA206/02/0346 GA ČR - Czech Science Foundation (CSF) GA206/05/0970 GA ČR - Czech Science Foundation (CSF) 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60050516 - BU-J (2005-2011) AV0Z50520514 - UMG-J (2005-2011) UT WOS 000263674500006 DOI 10.1007/s00606-008-0134-8 Annotation Nuclear sequences of ITS1-5.8S-ITS2 region of rDNA may be an important source of phylogenetically informative data provided that nrDNA is cloned and the character of sequence variation of clones is properly analysed. nrDNA of selected Taraxacum sections was studied to show sequence variation differences among diploid sexual, tetraploid sexual and polyploidy agamospermous species. We examined nucleotide characteristics, substitution pattern, secondary structure, and the phylogenetic utility of ITS1-5.8S-ITS2 from 301 clones of 32 species representing 11 sections. The most divergent sequences of ITS1 and 2 differed by 17.1 % and in 5.8S only by 3.7 %. The ITS1-5.8S-ITS2 characteristics, integrity and also stability of secondary structures confirmed that pseudogenes are not responsible for the above variation. The within-individual polymorphism of clones implies that the concerted evolution of ITS cistron of agamospermous polyploid Taraxacum is remarkably suppressed. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2009
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