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A first survey of the rye (Secale cereale) genome composition through BAC end sequencing of the short arm of chromosome 1R

SYSNO ASEP	0323090
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	A first survey of the rye (Secale cereale) genome composition through BAC end sequencing of the short arm of chromosome 1R
Title	První náhled do genomu žita (Secale cereale) pomocí sekvenování konců BAC klonů z krátkého ramene chromozómu 1R
Author(s)	Bartoš, Jan (UEB-Q)_{RID, ORCID} Paux, E. (FR) Kofler, R. (AT) Havránková, Miroslava (UEB-Q) Kopecký, David (UEB-Q)_{RID, ORCID} Suchánková, Pavla (UEB-Q)_RID Šafář, Jan (UEB-Q)_{RID, ORCID} Šimková, Hana (UEB-Q)_{RID, ORCID} Town, C.D. (US) Lelley, T. (AT) Feuillet, C. (FR) Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	BMC Plant Biology. - : BioMed Central - ISSN 1471-2229 Roč. 8, č. 95 (2008), s. 1-12
Number of pages	12 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	BAC libraries ; flow-sorted chromosomes
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GP521/06/P412 GA ČR - Czech Science Foundation (CSF)
	GD521/05/H013 GA ČR - Czech Science Foundation (CSF)
	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000259916300001
DOI	10.1186/1471-2229-8-95
Annotation	Background: Rye (Secale cereale L.) belongs to tribe Triticeae and is an important temperate cereal. It is one of the parents of man-made species Triticale and has been used as a source of agronomically important genes for wheat improvement. The short arm of rye chromosome 1 (1RS), in particular is rich in useful genes, and as it may increase yield, protein content and resistance to biotic and abiotic stress, it has been introgressed into wheat as the 1BL.1RS translocation. A better knowledge of the rye genome could facilitate rye improvement and increase the efficiency of utilizing rye genes in wheat breeding. Results: Here, we report on BAC end sequencing of 1,536 clones from two 1RS-specific BAC libraries. We obtained 2,778 (90.4%) useful sequences with a cumulative length of 2,032,538 bp and an average read length of 732 bp. These sequences represent 0.5% of 1RS arm. The GC content of the sequenced fraction of 1RS is 45.9%, and at least 84% of the 1RS arm consists of repetitive DNA. We identified transposable element junctions in BESs and developed insertion site based polymorphism markers (ISBP). Out of the 64 primer pairs tested, 17 (26.6%) were specific for 1RS. We also identified BESs carrying microsatellites suitable for development of 1RS-specific SSR markers. Conclusion: This work demonstrates the utility of chromosome arm-specific BAC libraries for targeted analysis of large Triticeae genomes and provides new sequence data from the rye genome and molecular markers for the short arm of rye chromosome 1.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2009

Number of the records: 1