Number of the records: 1

Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae

SYSNO ASEP	0322993
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvae
Title	Rychlé stanovení pohlaví u vajíček a larev obaleče jablečného Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae)
Author(s)	Vrbová, Iva (BC-A)_{RID, ORCID} Neven, L. G. (US) Bárcenas, N. M. (US) Gund, N. A. (DE) Dalíková, Martina (BC-A)_{RID, ORCID} Marec, František (BC-A)_{RID, ORCID}
Number of authors	6
Source Title	Journal of Applied Entomology. - : Wiley - ISSN 0931-2048 Roč. 133, č. 4 (2009), s. 249-261
Number of pages	13 s.
Language	eng - English
Country	DE - Germany
Keywords	Lepidoptera ; molecular markers ; period gene
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA206/06/1860 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000265035600003
DOI	10.1111/j.1439-0418.2008.01352.x
Annotation	Two different methods were tested to identify the sex of the early developmental stages of the codling moth with a WZ/ZZ (female/male) sex chromosome system. First, it was shown that the sex of larvae can be easily determined by the presence or absence of sex chromatin, which is formed by the W chromosome in interphase nuclei of females. Secondly, a new sexing technique was developed based on the molecular marker CpW2-EcoRI of the W chromosome. For this technique, a fragment of a codling moth gene, period (Cpper), was isolated and sequenced. Results of southern hybridization suggested that the Cpper gene is located on the Z chromosome. Then a multiplex PCR assay was developed, which co-amplified the CpW2-EcoRI sequence to identify the W chromosome and the Z-linked Cpper sequence, which served as a positive control of accurate processing of tested samples. The multiplex PCR provides an easy and rapid identification of the sex of embryos and early larval instars of the codling moth.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2009

Number of the records: 1