Number of the records: 1
Design and synthesis of bombykol analogues for probing pheromone-binding protein–ligand interactions
- 1.
SYSNO ASEP 0322756 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Design and synthesis of bombykol analogues for probing pheromone-binding protein–ligand interactions Title Návrh a syntéza analogů bombykolu pro studium interakcí ligandu s proteinem vázajícím feromon (PBP) Author(s) Mansurova, M. (DE)
Klusák, Vojtěch (UOCHB-X)
Nešněrová, P. (DE)
Muck, A. (DE)
Doubský, J. (DE)
Svatoš, Aleš (UOCHB-X) ORCID, RIDNumber of authors 6 Source Title Tetrahedron. - : Elsevier - ISSN 0040-4020
Roč. 65, č. 5 (2009), s. 1069-1076Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords bombykol ; pheromone ; binding protein ; nanoLC Subject RIV CC - Organic Chemistry CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000263218500015 DOI 10.1016/j.tet.2008.10.106 Annotation Fluorinated and thioanalogues of Bombyx mori female sex pheromones (bombykol) were designed according to the ab initio calculations. These analogues were synthesized using hydroboration and Sonogashira coupling strategy via (5E,7Z)-undecadien-1-ol as a common intermediate. A new binding assay based on nanoLC-linear ion trap ESI-MS for quantifying complexation of the B. mori pheromone-binding protein (BmPBP) with native and prepared analogues was developed. The dissociation constant (KD) of bombykol and analogue 4 was determined to be 2.1x10−6 M and 2.4x10−6 M, respectively. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2009
Number of the records: 1