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The purine derivative PI-55 blocks cytokinin action via receptor inhibition

SYSNO ASEP	0322293
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The purine derivative PI-55 blocks cytokinin action via receptor inhibition
Title	Purinový derivát PI-55 blokuje účinek cytokininů skrze inhibici receptorů
Author(s)	Spíchal, Lukáš (UEB-Q)_{RID, ORCID} Werner, T. (DE) Popa, Igor (UEB-Q) Riefler, M. (DE) Schmülling, T. (DE) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Source Title	FEBS Journal - ISSN 1742-464X Roč. 276, č. 1 (2009), s. 244-253
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	anticytokinin ; cytokinin ; cytokinin receptor
Subject RIV	ED - Physiology
R&D Projects	GP522/07/P197 GA ČR - Czech Science Foundation (CSF)
	LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000261683900022
DOI	10.1111/j.1742-4658.2008.06777.x
Annotation	We report the identification of the first known molecule antagonizing the activity of the plant hormone cytokinin at the receptor level. This compound, 6-(2-hydroxy-3-methylbenzylamino)purine, designated PI-55, is structurally related to cytokinin 6-benzylaminopurine, but substitutions at specific positions of the aromatic side chain strongly diminished its cytokinin activity and conferred antagonistic properties. PI-55 competitively inhibited the binding of the natural ligand trans-zeatin to the Arabidopsis cytokinin receptors CRE1/AHK4 and AHK3 and repressed induction of the cytokinin response gene ARR5:GUS. Genetic analysis revealed that CRE1/AHK4 is the primary target of PI-55. Cytokinin bioassays also demonstrated the anticytokinin effect of PI-55 in several other species. PI-55 accelerated the germination of Arabidopsis seeds and promoted the root growth and formation of lateral roots, thus phenocopying the known consequences of a lowered cytokinin status and demonstrating its potential to inhibit cytokinin perception in planta.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2009

Number of the records: 1