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Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous micropsheres
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SYSNO ASEP 0322285 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous micropsheres Title Extrakce PCR-ready DNA z bakteriofágů Staphylococcus aureus pomocí magnetických neporézních mikročástic s karboxylovými skupinami Author(s) Kahánková, J. (CZ)
Španová, A. (CZ)
Pantůček, R. (CZ)
Horák, Daniel (UMCH-V) RID, ORCID
Doškař, J. (CZ)
Rittich, B. (CZ)Source Title Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 877, č. 1 (2009), s. 599-602Number of pages 4 s. Language eng - English Country NL - Netherlands Keywords magnetic microspheres ; P(HEMA-co-EDMA) ; staphylococcus aureus Subject RIV CD - Macromolecular Chemistry R&D Projects GA203/05/2256 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000264225200003 DOI 10.1016/j.jchromb.2009.01.006 Annotation Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two newclinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCRready phage DNA can be isolated from small volumes of phage lysates (150 µl) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2009
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