Number of the records: 1

A new (14th) member of Borrelia burgdorferi sensu lato complex

SYSNO ASEP	0320267
Document Type	A - Abstract
R&D Document Type	Není vybrán druh dokumentu
Title	A new (14th) member of Borrelia burgdorferi sensu lato complex
Title	Nový (14.) člen komplexu Borrelia burgdorferi sensu lato
Author(s)	Oliver, J. (US) Rudenko, Natalia (BC-A)_{RID, ORCID} Golovchenko, Maryna (BC-A)_{RID, ORCID} Grubhoffer, Libor (BC-A)_{RID, ORCID}
Number of authors	4
Source Title	Clinical Microbiology and Infection - ISSN 1198-743X Roč. 14, S7 (2008), S110-S110
Number of pages	1 s.
Action	ECCMID /18./
Event date	19.04.2008-22.04.2008
VEvent location	Barcelona
Country	ES - Spain
Event type	EUR
Language	eng - English
Country	CA - Canada
Keywords	Borrelia burgdorferi s.l. complex ; new member ; multilocus sequence analysis
Subject RIV	EC - Immunology
R&D Projects	GA524/06/1479 GA ČR - Czech Science Foundation (CSF)
	LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
Annotation	Recently we studied 118 Borrelia isolates, cultured from samples collected from a variety of birds, rodents and ticks in 9 different localities of the southern region of the Unites States. A group of 16 isolates with unusual characteristics was found. The new methodology of multilocus sequence analysis (MLSA) that involved the study of rrf-rrl intergenic spacer region, rrs (16S RNA) gene, flagellin gene, ospA gene and p66 gene was used to clarify the taxonomic status of this new highly homogeneous group. All 13 recognized borrelia species were used as controls. The association of this species with Lyme disease group was confirmed by multiple results of MLSA. Unique RFLP pattern was discovered in the rrf-rrl intergenic spacer and flagellin gene. Unique signature nucleotides were allocated in the 16S rRNA. Phylogenetic analysis based on concatenated sequences of 5 loci showed all 16 isolates clustered together but separately from the other species of B. burgdorferi s.l. complex.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2009

Number of the records: 1