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A new (14th) member of Borrelia burgdorferi sensu lato complex
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SYSNO ASEP 0320267 Document Type A - Abstract R&D Document Type Není vybrán druh dokumentu Title A new (14th) member of Borrelia burgdorferi sensu lato complex Title Nový (14.) člen komplexu Borrelia burgdorferi sensu lato Author(s) Oliver, J. (US)
Rudenko, Natalia (BC-A) RID, ORCID
Golovchenko, Maryna (BC-A) RID, ORCID
Grubhoffer, Libor (BC-A) RID, ORCIDNumber of authors 4 Source Title Clinical Microbiology and Infection - ISSN 1198-743X
Roč. 14, S7 (2008), S110-S110Number of pages 1 s. Action ECCMID /18./ Event date 19.04.2008-22.04.2008 VEvent location Barcelona Country ES - Spain Event type EUR Language eng - English Country CA - Canada Keywords Borrelia burgdorferi s.l. complex ; new member ; multilocus sequence analysis Subject RIV EC - Immunology R&D Projects GA524/06/1479 GA ČR - Czech Science Foundation (CSF) LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Annotation Recently we studied 118 Borrelia isolates, cultured from samples collected from a variety of birds, rodents and ticks in 9 different localities of the southern region of the Unites States. A group of 16 isolates with unusual characteristics was found. The new methodology of multilocus sequence analysis (MLSA) that involved the study of rrf-rrl intergenic spacer region, rrs (16S RNA) gene, flagellin gene, ospA gene and p66 gene was used to clarify the taxonomic status of this new highly homogeneous group. All 13 recognized borrelia species were used as controls. The association of this species with Lyme disease group was confirmed by multiple results of MLSA. Unique RFLP pattern was discovered in the rrf-rrl intergenic spacer and flagellin gene. Unique signature nucleotides were allocated in the 16S rRNA. Phylogenetic analysis based on concatenated sequences of 5 loci showed all 16 isolates clustered together but separately from the other species of B. burgdorferi s.l. complex. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2009
Number of the records: 1