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A new (14th) member of Borrelia burgdorferi sensu lato complex

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    SYSNO ASEP0320267
    Document TypeA - Abstract
    R&D Document TypeNení vybrán druh dokumentu
    TitleA new (14th) member of Borrelia burgdorferi sensu lato complex
    TitleNový (14.) člen komplexu Borrelia burgdorferi sensu lato
    Author(s) Oliver, J. (US)
    Rudenko, Natalia (BC-A) RID
    Golovchenko, Maryna (BC-A) RID
    Grubhoffer, Libor (BC-A) RID
    Number of authors4
    Source TitleClinical Microbiology and Infection - ISSN 1198-743X
    Roč. 14, S7 (2008), S110-S110
    Number of pages1 s.
    ActionECCMID /18./
    Event date19.04.2008-22.04.2008
    VEvent locationBarcelona
    CountryES - Spain
    Event typeEUR
    Languageeng - English
    CountryCA - Canada
    KeywordsBorrelia burgdorferi s.l. complex ; new member ; multilocus sequence analysis
    Subject RIVEC - Immunology
    R&D ProjectsGA524/06/1479 GA ČR - Czech Science Foundation (CSF)
    LC06009 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z60220518 - PAU-O, BC-A (2005-2011)
    AnnotationRecently we studied 118 Borrelia isolates, cultured from samples collected from a variety of birds, rodents and ticks in 9 different localities of the southern region of the Unites States. A group of 16 isolates with unusual characteristics was found. The new methodology of multilocus sequence analysis (MLSA) that involved the study of rrf-rrl intergenic spacer region, rrs (16S RNA) gene, flagellin gene, ospA gene and p66 gene was used to clarify the taxonomic status of this new highly homogeneous group. All 13 recognized borrelia species were used as controls. The association of this species with Lyme disease group was confirmed by multiple results of MLSA. Unique RFLP pattern was discovered in the rrf-rrl intergenic spacer and flagellin gene. Unique signature nucleotides were allocated in the 16S rRNA. Phylogenetic analysis based on concatenated sequences of 5 loci showed all 16 isolates clustered together but separately from the other species of B. burgdorferi s.l. complex.
    WorkplaceBiology Centre (since 2006)
    ContactVáclava Lavičková, lavickova@hbu.cas.cz, Tel.: 387 775 889
    Year of Publishing2009