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Plant antigens cross-react with rat polyclonal antibodies against
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SYSNO ASEP 0318781 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plant antigens cross-react with rat polyclonal antibodies against Title Rostlinné antigeny křížově reagují s potkaními protilátkami proti peptidům konjugovaným s KLH Author(s) Oulehlová, Denisa (UEB-Q) RID
Hála, Michal (UEB-Q) RID, ORCID
Potocký, Martin (UEB-Q) RID, ORCID
Žárský, Viktor (UEB-Q) RID, ORCID
Cvrčková, F. (CZ)Source Title Cell Biology International. - : Elsevier - ISSN 1065-6995
Roč. 33, č. 1 (2009), s. 113-118Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords Keyhole limpet hemocyanin ; Rat antisera ; Synthetic peptides Subject RIV EB - Genetics ; Molecular Biology R&D Projects KJB600380601 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA204/05/0268 GA ČR - Czech Science Foundation (CSF) LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000261949000015 DOI 10.1016/j.cellbi.2008.10.003 Annotation Keyhole limpet hemocyanin (KLH)-conjugated peptides are routinely used to raise polyclonal antibodies for biochemical or immunolocalization studies. Rats are suitable for producing antisera against plant antigens as they often lack non-specific response towards plant materials. We attempted to obtain rat antisera against peptides derived from several plant proteins. However, most antisera recognized the same background KLH-related plant antigen (KRAP) in Arabidopsis and tobacco. We characterized KRAP with respect to size and cellular localization and examined possible antigen-specific reasons for the failure of most immunizations. We also found no reports of successful use of rat anti-KLH-peptide antibodies in plant studies. We thus believe that the rat-KLH:peptide system is poorly suited for production of antibodies, especially against plant antigens, and should be used with caution, if at all. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2009
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