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Ultrastructural localization of actin and actin-binding proteins in the nucleus

SYSNO ASEP	0318354
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Ultrastructural localization of actin and actin-binding proteins in the nucleus
Title	Ultrastrukturální lokalizace aktinu a aktin-vázajících proteinů v jádře
Author(s)	Dingová, Hana (UMG-J) Fukalová, Jana (UMG-J) Maninová, Miloslava (UMG-J) Philimonenko, Vlada (UMG-J) Hozák, Pavel (UMG-J)_{RID, ORCID}
Source Title	Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143 Roč. 131, č. 3 (2009), s. 425-434
Number of pages	10 s.
Language	eng - English
Country	DE - Germany
Keywords	nuclear actin ; ultrastructure ; actin–binding proteins
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000262697600012
Annotation	Nuclear actin plays an important role in such processes as chromatin remodeling, transcriptional regulation, RNA processing, and nuclear export. Recent research has demonstrated that actin in the nucleus probably exists in dynamic equilibrium between monomeric and polymeric forms, and some of the actin-binding proteins, known to regulate actin dynamics in cytoplasm, have been also shown to be present in the nucleus. In this paper, we present ultrastructural data on distribution of actin and various actin-binding proteins (alpha-actinin, filamin, p190RhoGAP, paxillin, spectrin, and tropomyosin) in nuclei of HeLa cells and resting human lymphocytes. Probing extracts of HeLa cells for the presence of actin-binding proteins also confirmed their presence in nuclei. We report for the first time the presence of tropomyosin and p190RhoGAP in the cell nucleus, and the spatial colocalization of actin with spectrin, paxillin, and alpha-actinin in the nucleolus.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2009

Number of the records: 1