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Antibody array analysis with label-based detection and resolution of protein size
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SYSNO ASEP 0318349 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Antibody array analysis with label-based detection and resolution of protein size Title Analýza založená na mnohočetném protilátkovém souboru, detekci značením a rozlišení velikosti proteinů Author(s) Wu, W. (NO)
Slaastad, H.S. (NO)
De la Rosa Carrillo, D. (NO)
Frey, T. (NO)
Tjonnfjord, G.E. (NO)
Boretti, E. (NO)
Aasheim, H.C. (NO)
Hořejší, Václav (UMG-J) RID
Lund-Jaohnsen, F. (NO)Source Title Molecular & Cellular Proteomics. - : Elsevier - ISSN 1535-9476
Roč. 8, č. 2 (2009), s. 245-257Number of pages 13 s. Language eng - English Country CA - Canada Keywords antibody array ; proteomics ; cell signalling Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50520514 - UMG-J (2005-2011) Annotation Proteins from different cellular compartments were labeled and separated by size exclusion chromatography into 20 fractions. The elution profiles were compiled to color maps across the size separation range (670-10kDa). A new solid phase designed for processing in microwell plates was developed to handle the large number of samples. Antibodies were bound to protein G-coupled microspheres surface-labeled with 300 combinations of four fluorescent dyes. Fluorescence from particle color codes and the protein label was measured by high speed flow cytometry. Cytoplasmic protein kinases, membrane proteins, cyclin-dependent kinases (cdks), cyclins and cdk-inhibitors, were analyzed; the results were compatible with their occurrence in complexes that vary with the cell cycle phase and subcellular localization. A two-dimensional platform extends the utility of antibody array analysis to studies of protein complexes. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2009
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