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Polymerase chain reaction multiplexing of microsatellites and single nucleotide polymorphism markers for quantitative trait loci mapping of wild house mice

SYSNO ASEP	0315471
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Polymerase chain reaction multiplexing of microsatellites and single nucleotide polymorphism markers for quantitative trait loci mapping of wild house mice
Title	Polymerázová řetězová reakce multiplexující mikrosatelity a SNP markery pro QTL mapování divokých myší
Author(s)	Kawalko, A. (PL) Dufková, Petra (UBO-W)_{RID, SAI} Wójcik, J. M. (PL) Piálek, Jaroslav (UBO-W)_{RID, ORCID, SAI}
Source Title	Molecular Ecology Resources - ISSN 1755-098X Roč. 9, č. 1 (2009), s. 140-143
Number of pages	4 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	house mouse ; microsatellites ; PCR multiplexing ; single nucleotide polymorphism markers
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA206/06/0955 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z60930519 - UBO-W (2005-2011)
UT WOS	000262678900025
DOI	10.1111/j.1755-0998.2008.02385.x
Annotation	We tested 96 microsatellites and 10 single nucleotide polymorphisms for their allelic distribution in two subspecies of the house mouse, Mus musculus musculus and M. m. domesticus. Sixty-two microsatellites discriminated strain-specific differences among nine wild-derived "musculus" and "domesticus" and three "classical" laboratory strains. For efficient genotyping, we optimized multiplex conditions using five microsatellites per polymerase chain reaction. All 10 single nucleotide polymorphisms were also optimized for simultaneous analysis in one reaction using SNaPshot multiplex. The uniform distribution of markers on autosomes and on the X chromosome makes these panels potentially useful tools for quantitative trait loci mapping of wild house mice.
Workplace	Institute of Vertebrate Biology
Contact	Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
Year of Publishing	2009

Number of the records: 1