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Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy

SYSNO ASEP	0314590
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy
Title	Oxidativní fosforylace a energetický status jsou reflektovány morfologií mitochondriální sítě u INS-1E a HEP-G2 buněk zobrazených 4Pi mikroskopií
Author(s)	Hlavatá, Lydie (FGU-C) Lessard, M. (US) Šantorová, Jitka (FGU-C)_RID Bewersdorf, J. (US) Ježek, Petr (FGU-C)_{RID, ORCID}
Source Title	Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728 Roč. 1777, 7-8 (2008), s. 834-846
Number of pages	13 s.
Language	eng - English
Country	NL - Netherlands
Keywords	4Pi microscopy ; 100 nm 3D imaging ; mitochondrial network
Subject RIV	EA - Cell Biology
R&D Projects	NR7917 GA MZd - Ministry of Health (MZ)
	NR9183 GA MZd - Ministry of Health (MZ)
	IAA500110701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GP303/05/P100 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000257696400039
DOI	10.1016/j.bbabio.2008.04.002
Annotation	Using 4Pi microscopy, 3D morphology changes of mitochondrial reticulum network responding to decreased oxidative phosphorylation have been observed in INS-1E and HEP-G2 cells at an unprecedented 100 nm level of detail. In INS-1E cells a single interconnected mitochondrion with a nearly constant 262+26 nm tubule diameter was found. With rotenone inhibiting respiration to 10%, disintegration into several reticula and numerous 300 nm spheres or short tubules was observed. De-energization by uncoupling additionally led to formation of rings and bulky cisternae of 1.4+0.4 μm diameter. Rotenone and uncoupler acted synergically in INS-1E cells and increased fusion (ongoing with fission) forming bowl-like shapes. In HEP-G2 cells fission partially ceased with FCCP plus rotenone. Thus we have revealed previously undescribed details for shapes upon mitochondrial disintegration and clearly demonstrate that high resolution 3D microscopy is required for visualization of mitochondrial network
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2009

Number of the records: 1