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Three-Dimensional Analysis of Norway Spruce Needle Anatomical Structure Using Stereology and Confocal Microscopy

SYSNO ASEP	0313464
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Three-Dimensional Analysis of Norway Spruce Needle Anatomical Structure Using Stereology and Confocal Microscopy
Title	Trojrozměrná analýza anatomické stavby jehlice smrku ztepilého pomocí stereologie a konfokální mikroskopie
Author(s)	Kubínová, Lucie (FGU-C)_{RID, ORCID} Lhotáková, Zuzana (FGU-C) Janáček, Jiří (FGU-C)_{RID, ORCID} Albrechtová, J. (CZ)
Source Title	Proceedings 8th multinational congress on microscopy. - České Budějovice : Czechoslovak microscopy society, 2007 / Nebesářová Jana ; Hozák Pavel - ISBN 978-80-239-9397-4 S. 479-480
Number of pages	2 s.
Action	Multinational Congress on Microscopy /8./
Event date	17.06.2007-21.06.2007
VEvent location	Prague
Country	CZ - Czech Republic
Event type	EUR
Language	eng - English
Country	CZ - Czech Republic
Keywords	spo2 ; spruce needle ; surface area ; stereology
Subject RIV	EF - Botanics
R&D Projects	IAA600110507 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
Annotation	We tested the effect of freezing Norway spruce needles on their anatomical structure as well as the effect of possible deformations due to the cutting of fresh needles by a hand microtome. Higher proportion of intercellular spaces in mesophyll in depths near to the surface of a physical section and lower mesophyll surface density was found and so the measurements should be restricted only to the middle region inside the physical section. All estimated mesophyll parameters did not show significant difference between fresh and frozen needles and therefore we conclude that freezing represents a suitable form of storage of sampled material for its stereological evaluation
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2009

Number of the records: 1