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Purification and characterization of a nitrilase from Fusarium solani O1

SYSNO ASEP	0313082
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Purification and characterization of a nitrilase from Fusarium solani O1
Title	Vyčištění a popis nitrilasy z houby Fusarium solani O1
Author(s)	Vejvoda, Vojtěch (MBU-M) Kaplan, Ondřej (MBU-M) Bezouška, Karel (MBU-M) Pompach, Petr (MBU-M)_{RID, ORCID} Šulc, Miroslav (MBU-M)_{RID, ORCID} Cantarella, M. (IT) Benada, Oldřich (MBU-M)_{ORCID, RID} Uhnáková, Bronislava (MBU-M) Rinágelová, Anna (MBU-M) Lutz-Wahl, S. (DE) Fischer, L. (DE) Křen, Vladimír (MBU-M)_{RID, ORCID} Martínková, Ludmila (MBU-M)_{RID, ORCID}
Source Title	Journal of Molecular Catalysis B-Enzymatic. - : Elsevier - ISSN 1381-1177 Roč. 50, 2-4 (2008), s. 99-106
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	nitrilase ; fusarium solani ; nitrile hydrolysis
Subject RIV	EE - Microbiology, Virology
R&D Projects	IAA500200708 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GA203/05/2267 GA ČR - Czech Science Foundation (CSF)
	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	OC D25.002 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000252487300009
DOI	10.1016/j.molcatb.2007.09.06
Annotation	An intracellular nitrilase was purified from a Fusarium solani O1 culture, in which the enzyme (up to 3000 U/L) was induced by 2-cyanopyridine. SDS-PAGE revealed one major band corresponding to a molecular weight of approximately 40 kDa. Peptide mass fingerprinting suggested a high similarity of the protein with the putative nitrilase from Gibberella moniliformis. Electron microscopy revealed that the enzyme molecules associated into extended rods. The enzyme showed the highest specific activities towards benzonitrile (156 U mg-1) and 4-cyanopyridine (203 U mg-1)
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2009

Number of the records: 1