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The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing
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SYSNO ASEP 0310881 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing Title Centrální sekvence CpG ostrova chrání vektory odvozené od ptačích sarkomových a leukosových virů před transkripčním umlčením Author(s) Šenigl, Filip (UMG-J) RID
Plachý, Jiří (UMG-J) RID
Hejnar, Jiří (UMG-J) RIDSource Title Journal of Virology - ISSN 0022-538X
Roč. 82, č. 16 (2008), s. 7818-7827Number of pages 10 s. Language eng - English Country US - United States Keywords anti-methylation protection ; retroviral vector ; CpG island Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/05/0939 GA ČR - Czech Science Foundation (CSF) GA523/07/1171 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000258166500007 Annotation Rous sarcoma virus and retroviral vectors derived from it suffer from progressive transcriptional silencing in mammalian cells. The insertion of the short core sequence of a CpG island into the long terminal repeat of such vector led to efficient protection of the integrated vector from silencing and gradual CpG methylation in rodent and human cells. Individual cell clones with IE-modified reporter vectors display high levels of reporter expression for a sustained period and without substantial variegation in the cell culture. The presence of Sp1 binding sites is important for the protective effect of IE, but at least some part of the entire antisilencing capacity is maintained in IE with mutated Sp1 sites. We suggest that this strategy of antisilencing protection by the CpG island core element may prove generally useful in retroviral vectors. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2009
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