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Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride
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SYSNO ASEP 0309822 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Native, denatured and reduced BSA enhancement of chronopotentiometric peak H by guanidinium chloride Title Nativní, denaturovaný a redukovaný BSA. Zvýraznění chronopotenciometrického píku H guanidinium chloridem Author(s) Ostatná, Veronika (BFU-R) RID, ORCID
Paleček, Emil (BFU-R) RID, ORCIDSource Title Electrochimica acta. - : Elsevier - ISSN 0013-4686
Roč. 53, č. 11 (2008), s. 4014-4021Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords constant current chronopotentiometry ; protein denaturation ; guanidinium chloride Subject RIV AQ - Safety, Health Protection, Human - Machine R&D Projects GA301/07/0490 GA ČR - Czech Science Foundation (CSF) GP202/07/P497 GA ČR - Czech Science Foundation (CSF) IAA500040513 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50040507 - BFU-R (2005-2011) AV0Z50040702 - BFU-R (2007-2013) UT WOS 000254728900036 DOI 10.1016/j.electacta.2007.10.035 Annotation It was shown that using constant current chronopotentiometric stripping peak H, minute amounts of denatured and reduced bovine serum albumin (BSA) can be easily discriminated from native BSA. Peak H, which is due to catalytic hydrogen evolution, is greatly enhanced in the presence of non-denaturing concentrations of guanidinium chloride. The course of BSA reduction and denaturation can be followed and traces of the damaged protein can be detected in native BSA samples. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2009
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