Mouse consomic strains: Exploiting genetic divergence between Mus m. musculus and Mus m. domesticus subspecies

Gregorová, Soňa; Divina, Petr; Štorchová, Radka; Trachtulec, Zdeněk; Fotopulosová, Vladana; Svenson, K.L.; Donahue, K.L.; Paigen, B.; Forejt, Jiří

Number of the records: 1

Mouse consomic strains: Exploiting genetic divergence between Mus m. musculus and Mus m. domesticus subspecies

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SYSNO ASEP	0306624
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mouse consomic strains: Exploiting genetic divergence between Mus m. musculus and Mus m. domesticus subspecies
Title	Myší konsomické kmeny: Využití genetické rozdílnosti mezi poddruhy Mus m. musculus a Mus m. domesticus
Author(s)	Gregorová, Soňa (UMG-J) Divina, Petr (UMG-J) Štorchová, Radka (UMG-J) Trachtulec, Zdeněk (UMG-J)_{RID, ORCID} Fotopulosová, Vladana (UMG-J) Svenson, K.L. (US) Donahue, K.L. (US) Paigen, B. (US) Forejt, Jiří (UMG-J)_{RID, ORCID}
Source Title	Genome Research. - : Cold Spring Harbor Laboratory Press - ISSN 1088-9051 Roč. 18, č. 3 (2008), s. 509-515
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	complex trait loci (QTLs) ; chromosome substitution strains ; PWD/Ph
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA301/07/1264 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000253766700017
Annotation	Consomic (chromosome substitution) strains (CSs) represent the most recent addition to the mouse genetic resources aimed to geneticaly analyze complex trait loci (QTLs). In this study, we report the development of a set of 28 mouse inter-subspecific CSs. In each CS, we replaced a single chromosome of the C57BL/6J (B6) inbred strain (mostly Mus m. domesticus) with its homolog from the PWD/Ph inbred strain of the Mus m. musculus subspecies. These two progenitor subspecies diverged less than 1 million years ago and accumulated a large number of genetic differences that constitute a rich resource of genetic variation for QTL analyses. A genome-wide scan of 965 DNA markers revealed 99.87% purity of the B6 genetic background. Thirty-three nonsynonymous substitutions were uncovered in the protein-coding regions of the mitochondrial DNA of the B6.PWD-mt conplastic strain. A pilot phenotyping experiment project revealed a high number of variations among B6.PWD consomics.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2009

Number of the records: 1