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Tumour-inhibitory and antimetastatic effects of IL-2 in mice carrying MHC class I- tumours of HPV16 origin
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SYSNO ASEP 0191662 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Tumour-inhibitory and antimetastatic effects of IL-2 in mice carrying MHC class I- tumours of HPV16 origin Author(s) Indrová, Marie (UMG-J) RID
Bubeník, Jan (UMG-J)
Mikyšková, Romana (UMG-J) RID
Vonka, V. (CZ)
Šmahel, M. (CZ)
Žák, R. (CZ)
Šímová, Jana (UMG-J) RID
Bieblová, Jana (UMG-J)
Mendoza, Luis (UMG-J)
Jandlová, Táňa (UMG-J)Source Title International Journal of Oncology. - : Spandidos Publications - ISSN 1019-6439
Roč. 2002, č. 20 (2002), s. 643-646Number of pages 4 s. Language eng - English Country GR - Greece Keywords HPV16, IL-2 ; tumour vaccines ; gene therapy Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z5052915 - UMG-J Annotation Oncogenic, moderately immunogenic, MHC class I- and class II-, B7(-), MK16/l/III ABC (MK16) cells were previously established by co-transfection of HPV16 E6/E7 and activated H-ras oncogene DNA into C57BL/6 kidney cells. Subcutaneous transplantation of these cells produced progressively growing local neoplasms which metastasized spontaneously to lungs and lymph nodes. The MK16 cells were implanted into syngeneic mice and used to examine whether the tumour lacking the signal molecules required for the induction of and sensitivity to T cell immunity is susceptible to local IL-2 treatment and IL-2 gene therapy. Peritumoural administration of human rIL-2 or murine IL-2 gene-modified MK16 tumour vaccine inhibited growth of subcutaneous MK16 tumour transplants and reduced the number of their lung metastases. These results indicate that experimental tumours which are MHC class I- and mimick in this respect a high proportion of human HPV16-associated carcinomas are suitable for IL-2 treatment. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2004
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