Immobilization of L-glyceryl phosphorylcholine: isolation of phosphorylcholine-binding proteins from seminal plasma
1.
SYSNO ASEP
0191443
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Immobilization of L-glyceryl phosphorylcholine: isolation of phosphorylcholine-binding proteins from seminal plasma
Author(s)
Liberda, J. (CZ) Maňásková, Pavla (UMG-J) Švesták, M. (CZ) Jonáková, Věra (UMG-J) Tichá, M. (CZ)
Source Title
Journal of Chromatography-B. - : Elsevier BV
- ISSN 0378-4347
Roč. 770, 1-2 (2002), s. 101-110
Number of pages
10 s.
Language
eng - English
Country
IE - Ireland
Keywords
L-glyceryl phosphorylcholine ; proteins
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
GA303/99/0357 GA ČR - Czech Science Foundation (CSF)
GV524/96/K162 GA ČR - Czech Science Foundation (CSF)
CEZ
AV0Z5052915 - UMG-J
MSM 113100001
Annotation
The preparation of an affinity sorbent containing immobilized L-glyceryl phosphorylcholine for affinity chromatography of phosphorylcholine-binding proteins from bear, bull and human seminal plasmas is described. Adsorbed phosphorylcholine-binding proteins were characterized by SDS-PAGE and RP HPLC. In human seminal plasma no phosphorylcholine-binding proteins were found.
