Y chromosome and vimentin used to trace the fate of allogeneic keratinocytes delivered to the wound by the recombined human/pig skin
1.
SYSNO ASEP
0191224
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Y chromosome and vimentin used to trace the fate of allogeneic keratinocytes delivered to the wound by the recombined human/pig skin
Author(s)
Pokorná, Eva (UMG-J) Brož, L. (CZ) Veselý, Pavel (UMG-J) Matoušková, Eva (UMG-J)
Source Title
Folia Biologica. - : Univerzita Karlova v Praze
- ISSN 0015-5500
Roč. 47, č. 4 (2001), s. 128-134
Number of pages
7 s.
Language
eng - English
Country
CZ - Czech Republic
Keywords
allogeneic keratinocytes ; xenodermis ; Y-chromosome FISH
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
IZ4368 GA MZd - Ministry of Health (MZ)
NK6126 GA MZd - Ministry of Health (MZ)
Annotation
Allogeneic keratinocytes cultured on cell-free pig dermisstimulate wound healing when applied with the epidermal layerfacing the wound. Male keratinocytes were grafted into donorsites of three female patients. Biopsies were taken on days 4, 6 and 9. The Y-chromosome was found (using the fluorescence .I.in situ ./I.hybridization) in donor sites on day 4 and 6, but not on day 9. Conclusion: Allongeneic keratinocytes temporarily "take" to the wound and are replaced by the patient`s cells after about one week.
