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Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag, Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles

SYSNO ASEP	0181618
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag, Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles
Author(s)	Humpolíčková, J. (CZ) Procházka, K. (CZ) Hof, Martin (UFCH-W)_{RID, ORCID} Tuzar, Zdeněk (UMCH-V)_RID Špírková, Milena (UMCH-V)_{RID, ORCID}
Source Title	Langmuir. - : American Chemical Society - ISSN 0743-7463 Roč. 19, - (2003), s. 4111-4119
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	fluorescence ; spectroscopy
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	LN00A032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA203/01/0536 GA ČR - Czech Science Foundation (CSF)
	GA203/01/0735 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z4050913 - UMCH-V
	AV0Z4040901 - UFCH-W
Annotation	Amphiphilic multimolecular block copolymer micelles of polystyrene-block-poly(methacrylic acid), PS-PMA and polystyrene-block-poly(ethylene oxide), PS-PEO, were investigated by fluorescence correlation spectroscopy (FCS) in aqueous media using octadecylrhodamine B (ORB) as a micelle-specific probe, together with UV-Vis absorption and other fluorescence techniques (steady-state spectroscopy and depolarization and time-resolved fluorescence decay measurements). The aim of the study was to show that the fluorescence techniques alone provide sufficient information on the micellar system, allowing thus for the fully conclusive study. Other techniques, such as static and quasielastic light scattering (SLS and QELS) and atomic force microscopy (AFM) were used just for comparison in order to prove the reliability of FCS results and to estimate their accuracy. Behavior of ORB in aqueous solutions, the association-dependent changes in its UV-Vis and fluorescence spectra and its binding kinetics.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2004

Number of the records: 1