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Isolation of melatonin by immunoaffinity chromatography

SYSNO ASEP	0172270
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Isolation of melatonin by immunoaffinity chromatography
Author(s)	Rolčík, Jakub (UEB-Q) Lenobel, René (UEB-Q)_ORCID Siglerová, Věra (UEB-Q) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Source Title	Journal of Chromatography-B. - : Elsevier BV - ISSN 0378-4347 Roč. 25, č. 1 (2002), s. 9-15
Number of pages	7 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Melatonin
Subject RIV	CE - Biochemistry
R&D Projects	OC 844.10 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA301/02/0475 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z5038910 - UEB-Q
Annotation	A single-step, highly specific and easy-to-use method was developed for isolation and purification of melatonin from complex biological matrices. Polyclonal antibodies highly specific against melatonin (with cross-reactivities with related compounds below 0.02%, except for 6-hydroxymelatonin) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of immunoaffinity gel. Melatonin recovery by the immunoaffinity method was ~95%, allowing single-step processing of samples prior to electrospray HPLCůMS analysis (with detection limit 10 fmol). The method was successfully used for determining melatonin in human serum and turned out to be better than the non-specific solid-phase extraction published earlier.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2003

Number of the records: 1