Number of the records: 1

Aromatic cytokinins in micropropagated potato plants

SYSNO ASEP	0172256
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Aromatic cytokinins in micropropagated potato plants
Author(s)	Baroja, F. E. (ES) Aguirreolea, J. (ES) Martínková, Hana (UEB-Q) Hanuš, Jan (UEB-Q) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Source Title	Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428 Roč. 40, č. 3 (2002), s. 217-224
Number of pages	8 s.
Language	eng - English
Country	FR - France
Keywords	Acclimatization ; Aromatic cytokinins ; Micropropagation
Subject RIV	CE - Biochemistry
R&D Projects	OC 844.10 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA301/02/0475 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z5038910 - UEB-Q
Annotation	Endogenous cytokinins were studied in three micropropagated Solanum tuberosum L. cultivars (Kennebec, Turia and Jaerla) differing in survival after transplanting. Leaf and stem cytokinins were determined both in vitro and 10 d after being transferred to ex vitro conditions by a combination of high-performance liquid chromatography and enzyme-linked immunosorbent assay. Nine aromatic and nine isoprenoid type cytokinins were identified. Higher levels of total cytokinins mainly aromatics (92%) were detected in Kennebec, the cultivar showing better in vitro growth and 99% survival. On the contrary, a predominance of isoprenoid cytokinins (up to 57%) was observed after transplanting in Jaerla, the cultivar showing lower viability. Significant survival improvement was obtained in the Jaerla cultivar after addition to the culture medium of the aromatic cytokinin meta-topolin riboside (mTR). We also report here isolation and identification of this cytokinin by several sophisticated techniques including mTR-specific immunoaffinity chromatography, diode-array high-performance liquid chromatography (HPLC), and gas chromatographyůmass spectrometry of permethylated HPLC fractions. The occurrence of the aromatic cytokinins in potato plants is described for the first time.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2003

Number of the records: 1