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Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells

SYSNO ASEP	0172240
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells
Author(s)	Dobrev, Petre (UEB-Q)_{RID, ORCID} Motyka, Václav (UEB-Q)_{RID, ORCID} Gaudinová, Alena (UEB-Q)_ORCID Malbeck, Jiří (UEB-Q)_{RID, ORCID} Trávníčková, Alena (UEB-Q)_ORCID Kamínek, Miroslav (UEB-Q)_{RID, ORCID} Vaňková, Radomíra (UEB-Q)_{RID, ORCID}
Source Title	Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428 Roč. 40, - (2002), s. 333-337
Number of pages	5 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	Cis zeatin ; Cytokinins ; Cytokinin oxidase
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA522/99/1130 GA ČR - Czech Science Foundation (CSF)
	GV206/96/K188 GA ČR - Czech Science Foundation (CSF)
	IAA6038002 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z5038910 - UEB-Q
Annotation	Changes in levels of endogenous cytokinins and activity of cytokinin oxidase (EC 1.4.3.6) were followed during the cell cycle progression in large-scale (ca. 30 g FW) synchronized culture of the tobacco cell line BY-2. Synchronization was induced either by 4 mM hydroxyurea or 15 iM aphidicolin alone (a single block of the cell cycle at G,/S) or in combination with 1 uM oryzalin (double blocking at G,/S and Gz/M). Up to 46% synchrony was obtained. Two distinct cytokinin peaks were detected at 0-2 h and 8.5-9 h after the release from the first block coinciding with the beginning of the S phase and with the onset of mitosis, respectively. Interestingly, as identified by MS/MS, very sharp and transient premitotic peak of cytokinins contained, in addition to fra/u-zeatin, also considerable amounts of cu-zeatin and zeatin-0-glucoside. Cytokinin oxidase activity exhibited conspicuous maximum parallel to the first peak of cytokinin accumulation and deep minimum corresponding to the culmination of mitotic index. Possible roles of zeatin cis-trans isomerisation, glucosylation and degradation by cytokinin oxidase in regulation of cytokinin levels during the cell cycle progression are discussed.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2003

Number of the records: 1