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Fast sex identification in wild mammals using PCR amplification of the Sry gene
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SYSNO ASEP 0164504 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fast sex identification in wild mammals using PCR amplification of the Sry gene Author(s) Bryja, Josef (UBO-W) RID, SAI, ORCID
Konečný, A. (CZ)Source Title Folia zoologica. - : Ústav biologie obratlovců AV ČR, v. v. i. - ISSN 0139-7893
Roč. 52, č. 3 (2003), s. 269-274Number of pages 6 s. Language eng - English Country CZ - Czech Republic Keywords sex identification ; Sry gene ; rodents Subject RIV EG - Zoology R&D Projects GA524/01/1316 GA ČR - Czech Science Foundation (CSF) GP206/02/P068 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z6093917 - UBO-W UT WOS 000185864800005 EID SCOPUS 0142091382 Annotation Several primer sets were described for PCR amplification of the Sry gene that became very suitable method for sex identification in mammals. We tested applicability of two primer sets for sex identification in one bat and 12 rodent species from Central Europe. Rapid DNA extraction with non-ionic detergents, which is very suitable for large-scale population studies, was used. Primers SRY-A from Pomp et al. (1995) did not amplify Sry fragment in males of Myotis myotis, four species of Microtus, and Clethrionomys glareolus, while amplification was successful in four species of Apodemus, two species of Mus, and Sciurus vulgaris. On the other hand, primers from Sanchez et al. (1996) gave clear Sry band in males of all tested species, only with one exception, i.e. male of Sciurus vulgaris. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2004
Number of the records: 1