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Chloride present in biological samples as a tool for enhancement of sensitivity in capillary zone electrophoretic analysis of anionic trace analytes
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SYSNO ASEP 0162494 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Chloride present in biological samples as a tool for enhancement of sensitivity in capillary zone electrophoretic analysis of anionic trace analytes Author(s) Křivánková, Ludmila (UIACH-O) RID
Pantůčková, Pavla (UIACH-O) RID
Gebauer, Petr (UIACH-O) RID, ORCID
Boček, Petr (UIACH-O) RID
Caslavska, J. (CH)
Thormann, W. (CH)Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 24, č. 3 (2003), s. 505-517Number of pages 13 s. Language eng - English Country DE - Germany Keywords acetoacetate ; capillary zone electrophoresis ; chloride stacking effects Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA203/02/0023 GA ČR - Czech Science Foundation (CSF) GA203/01/0401 GA ČR - Czech Science Foundation (CSF) IAA4031103 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z4031919 - UIACH-O Annotation For stacking and separation of anionic trace analytes in biological samples, the presence of chloride is shown to be important to balance out effects of other macrocomponents that act against isotachophoretic stacking. Having acetoacetate, malate, citrate and some drug metabolites in untreated human serum samples, the stacking mechanism of these compounds in an electrolyte system comprising 5 mM mandelic acid + e-aminocaproic acid, pH 3.8, is explained. Insight into the separation process is given both with computer simulations and experiments. For selected analytes, the effect of chloride concentration on quantitative evaluation, sensitivity and limit of detection is demonstrated as well. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2004
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