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Different methods of membrane domains isolation result in similar 2-D distribution patterns of membrane domain proteins

SYSNO ASEP	0142571
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Different methods of membrane domains isolation result in similar 2-D distribution patterns of membrane domain proteins
Author(s)	Matoušek, Petr (FGU-C) Hodný, Zdeněk (UEM-P) Švandová, I. (CZ) Svoboda, Petr (FGU-C)_{RID, ORCID}
Source Title	Biochemistry and Cell Biology - ISSN 0829-8211 Roč. 81, č. 6 (2003), s. 365-372
Number of pages	8 s.
Language	eng - English
Country	CA - Canada
Keywords	membrane domain ; G protein ; two-dimensional electrophoresis ; GPI-ancored proteins
Subject RIV	CE - Biochemistry
R&D Projects	LN00A026 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z5011922 - FGU-C
	MSM 113100003
	AV0Z5039906 - UEM-P
Annotation	The present work compared three different procedures (drastic homogenisation, detergent extraction and alkaline treatment) of membrane domain isolation. Protein composition of membrane domains prepared by different procedures was very similar.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2004

Number of the records: 1