Number of the records: 1  

Confocal and two-photon microscopy: foundations, applications, and advances

  1. 1.
    SYSNO ASEP0142299
    Document TypeM - Monograph Chapter
    R&D Document TypeMonograph Chapter
    TitleStereological methods for estimating geometrical parameters of microscopic structure by three-dimensional imaging
    Author(s) Kubínová, Lucie (FGU-C) RID, ORCID
    Janáček, Jiří (FGU-C) RID, ORCID
    Krekule, Ivan (FGU-C)
    Source TitleConfocal and two-photon microscopy: foundations, applications, and advances, 15 / Diaspro A.. - New York : WileyLiss, 2002 - ISBN 0-471-40920-0
    Pagess. 299-332
    Number of pages34 s.
    Languageeng - English
    CountryUS - United States
    Keywordsthree-dimensional imagine ; confocal microscopy
    Subject RIVEA - Cell Biology
    R&D ProjectsIAA5011810 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    GA304/01/0257 GA ČR - Czech Science Foundation (CSF)
    GA304/99/0680 GA ČR - Czech Science Foundation (CSF)
    ME 184 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z5011922 - FGU-C
    AnnotationMethods for estimating number, length, surface area and volume of microscopical structures, based on evaluation of three-dimensional image data obtained by confocal microscopy, are presented: (i) interactive unbiased stereological methods using virtual spatial grids generated by a computer applied to thick slices of arbitrary orientation; (ii) automatic digital methods based on image processing; (iii) automatic digital methods based on surface triangulation. The application of all methods, using our programs and software modules, is shown on practical examples. The methods are compared from the point of view of their applicability, efficiency and precision.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2003

Number of the records: 1  

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