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Confocal and two-photon microscopy: foundations, applications, and advances
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SYSNO ASEP 0142299 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Stereological methods for estimating geometrical parameters of microscopic structure by three-dimensional imaging Author(s) Kubínová, Lucie (FGU-C) RID, ORCID
Janáček, Jiří (FGU-C) RID, ORCID
Krekule, Ivan (FGU-C)Source Title Confocal and two-photon microscopy: foundations, applications, and advances, 15 / Diaspro A.. - New York : WileyLiss, 2002 - ISBN 0-471-40920-0 Pages s. 299-332 Number of pages 34 s. Language eng - English Country US - United States Keywords three-dimensional imagine ; confocal microscopy Subject RIV EA - Cell Biology R&D Projects IAA5011810 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA304/01/0257 GA ČR - Czech Science Foundation (CSF) GA304/99/0680 GA ČR - Czech Science Foundation (CSF) ME 184 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z5011922 - FGU-C Annotation Methods for estimating number, length, surface area and volume of microscopical structures, based on evaluation of three-dimensional image data obtained by confocal microscopy, are presented: (i) interactive unbiased stereological methods using virtual spatial grids generated by a computer applied to thick slices of arbitrary orientation; (ii) automatic digital methods based on image processing; (iii) automatic digital methods based on surface triangulation. The application of all methods, using our programs and software modules, is shown on practical examples. The methods are compared from the point of view of their applicability, efficiency and precision. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2003
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