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Temperature effect on carbachol-induced depression of spontaneous quantal transmitter release in frog neuromuscular junction
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SYSNO ASEP 0142279 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Temperature effect on carbachol-induced depression of spontaneous quantal transmitter release in frog neuromuscular junction Author(s) Strunsky, E. G. (RU)
Borisover, M. D. (IL)
Nikolsky, E. E. (RU)
Vyskočil, František (FGU-C) RIDSource Title Neurochemical Research. - : Springer - ISSN 0364-3190
Roč. 26, 8-9 (2001), s. 891-897Number of pages 7 s. Language eng - English Country US - United States Keywords carbachol ; temperature ; acetylcholine release Subject RIV ED - Physiology R&D Projects IAA7011902 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) OK 267 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z5011922 - FGU-C Annotation The effects of carbachol (CCh) on the frequency (f) of the miniature endplate potentials were tested at temperatures between 5 and 30 degrees C. Higher CCh concentrations, 1 x 10(-5) and 5 x 10(-6) M, reduced the f to 60 and the temperature dependence was negligible. However, an inverse temperature dependence was found when low concentrations 3 x 10(-7) and 6 x 10(-7) M were applied. The depression of f was 40-50 in 5-10 degrees C but only 10-20 of the control in the 25 and 30 degrees C. During application of CCh, the new steady of f was reached at temperatures between 5 and 30 degrees C within 17-20 min (Q10 = 1.07). Much greater temperature dependence of recovery was observed during washing out CCh (Q10 = 1.6). The temperature-independence of the steady state effects of CCh, good agreement with Langmuir adsorption-desorption theory and non-steady kinetics indicate that physical rather than receptor-mediated events are responsible for the depression of f. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2002
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