Number of the records: 1

Heat-inducible transgenic expression in the silkmoth Bombyx mori

SYSNO ASEP	0135886
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Heat-inducible transgenic expression in the silkmoth Bombyx mori
Author(s)	Uhlířová, Miroslava (ENTU-I) Asahina, Masako (PAU-O) Riddiford, L. M. (US) Jindra, Marek (ENTU-I)
Source Title	Development Genes and Evolution - ISSN 0949-944X Roč. 212, - (2002), s. 145-151
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	Germline transformation ; piggyBac transposon
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	IAB5007002 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z5007907 - ENTU-I, BC-A
Annotation	We report successful germline transformation of the silkmoth Bombyx mori with the piggyBas transposon. Besides the Pax-6driven GFP selectable marker, we inserted into the Bombyx genome a heat-inducible hsp 70 cassette from Drosophila. For the first time we show that a transgene can be induced in vivo by heat shock in a non-drosophilid insect, establishing a system for future transgenic studies.
Workplace	Institute of Entomology (until 2005)
Contact	Helena Musilová, musilova@entu.cas.cz, Tel.: 387 775 216
Year of Publishing	2003

Number of the records: 1