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Microbiotests of cyanobacterial toxins screening
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SYSNO ASEP 0129231 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Microbiotests of cyanobacterial toxins screening Author(s) Maršálek, Blahoslav (BU-J) ORCID, SAI
Bláha, L. (CZ)Source Title New microbiotests for routine toxicity screening and biomonitoring / Persoone G. ; Janssen C. ; De Coen W.. - New York : Kluwer Academic / Plenum Publishers, 2000 - ISBN 0-306-46406-3 Pages s. 519-525 Number of pages 7 s. Action International Symposium New Microbiotests for Routine Toxicity Screening and Biomonitoring Event date 01.06.1998-03.06.1998 VEvent location Brno Country CZ - Czech Republic Language eng - English Country US - United States Keywords toxicity. ; microbiotests. ; cyanobacteria. Subject RIV EF - Botanics R&D Projects KSK2005601 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Annotation Microbioassays are typical non-specific tests. The toxicity signal results from the whole spectrum of consequences, especially, when phytoplankton biomass is tested for toxins. Embryotoxicity, genotoxicity and mutagenity, immunotoxicity ,cytotoxicity and other toxic cyanobacterial metabolites can be tested by specific miniaturised biosystems. Typical problems occur when general ecotoxicity tests are used for the pre-screening of cyanotoxins. Bacterial tests were found as quite unreliable because no correlation with dose response of main toxins was found. Extracts of cyanobacterial biomass provide a number of interpretation problems also for crustacean and protozoan species, which were found to be sensitive for cyanotoxins. We developed system for the fractionation of biomass. The fraction of toxins, pigments and other metabolites are separed by C-18 cartridges and molecular sieves (10.000MV) and are tested by biological assays. Mortality of Thamnocephalus or Daphnia in crude cyanobacterial extracts can be caused for example by the lack of oxygen or other factors which have no consequences with the cyanotoxins. However, microbiotests using fractionated biomass can be used as pre-screening of cyanotoxins in drinking water supply or recreational water bodies.Microbiotests for cyanotoxins toxicity pre-screening. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2002
Number of the records: 1