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Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression
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SYSNO ASEP 0109747 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression Title Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression Author(s) Galuszka, P. (CZ)
Frébortová, Jitka (UEB-Q) RID, ORCID
Werner, T. (DE)
Yamada, M. (JP)
Strnad, Miroslav (UEB-Q) RID, ORCID
Schmülling, T. (DE)
Frébort, I. (CZ)Source Title European Journal of Biochemistry - ISSN 0014-2956
Roč. 271, č. 20 (2004), s. 3990-4002Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords cereals ; cloning ; cytokinin oxidase/dehydrogenase Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z5038910 - UEB-Q Annotation The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. Transformation of both genes into Arabidopsis and tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. Additional cloning of two gene fragments, and an in silico search in the public expressed sequence tag clone databases, revealed the presence of at least 13 more members of the CKX gene family in barley and wheat. The expression of three selected barley genes was analyzed by RT-PCR and found to be organ-specific with peak expression in mature kernels. One barley CKX (HvCKX2) was characterized in detail after heterologous expression in tobacco. Interestingly, this enzyme shows a pH optimum at 4.5 and a preference for cytokinin ribosides as substrates, which may indicate its vacuolar targeting. Different substrate specificities, and the pH profiles of cytokinin-degrading enzymes extracted from different barley tissues, are also presented. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2005
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