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Microbiological aspects of determination of trichloroacetic acid in soil
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SYSNO ASEP 0109150 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Microbiological aspects of determination of trichloroacetic acid in soil Title Microbiological aspects of determination of trichloroacetic acid in soil Author(s) Matucha, Miroslav (MBU-M)
Gryndler, Milan (MBU-M) RID, ORCID
Uhlířová, H. (CZ)
Fuksová, K. (CZ)
Rohlenová, Jana (UEB-Q)
Forczek, Sándor (UEB-Q) RID, ORCID
Schröder, P. (DE)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 49, č. 2 (2004), s. 117-122Number of pages 6 s. Language eng - English Country CZ - Czech Republic Keywords NORWAY SPRUCE/SOIL-SYSTEM ; HEADSPACE GAS-CHROMATOGRAPHY ; BIODEGRADATION Subject RIV GK - Forestry R&D Projects GA522/02/0874 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5038910 - UEB-Q Annotation Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at -20 degreesC and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2005
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