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Different nuclear localization of aberrant and normal chromosome 8 homologues in intact of NaBt differentiated HT-29 cells
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SYSNO ASEP 0108492 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Different nuclear localization of aberrant and normal chromosome 8 homologues in intact of NaBt differentiated HT-29 cells Title Rozdílná jaderná lokalizace aberantních a normálních homologů chromosomu 8 v intaktních nebo NaBt diferencovaných buňkách HT-29 Author(s) Harničarová, Andrea (BFU-R) ORCID
Bártová, Eva (BFU-R) ORCID
Kozubek, Stanislav (BFU-R) RIDSource Title Biophysics of the Genome: Proceedings of the Workshop Held at Brno. - Brno : Masarykova univerzita v Brně, 2004 / Kozubek S. ; Kozubek M. - ISBN 80-210-3560-9 Pages s. 24-27 Number of pages 4 s. Action Biophysics of the Genome Event date 12.10.2004-13.10.2004 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords chromosome 8 homologues ; NaBt differentiated HT-29 cells Subject RIV BO - Biophysics R&D Projects IAA5004306 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IBS5004010 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KSK5052113 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA202/04/0907 GA ČR - Czech Science Foundation (CSF) NC6987 GA MZd - Ministry of Health (MZ) ME 565 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z5004920 - BFU-R Annotation An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2005
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