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Transcription Factor NF-IL6 (C/EBPbeta) Activates the Expression of the Mouse MHC Class I H2-Kb Gene in Response to TNF-alpha via the Intragenic Downstream Regulatory Element

SYSNO ASEP	0105301
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Transcription Factor NF-IL6 (C/EBPbeta) Activates the Expression of the Mouse MHC Class I H2-Kb Gene in Response to TNF-alpha via the Intragenic Downstream Regulatory Element
Title	Transkripční faktor NF-IL6 (C/EBPbeta) aktivuje expresi myšího genu MHC 1. třídy H2-Kb v reakci na TNF-alfa pomocí regulačního elementu nacházejícího se uvnitř genu po směru transkripce
Author(s)	Hatina, J. (CZ) Jansa, Petr (UMG-J)_RID Reischig, J. (CZ)
Source Title	Journal of Interferon and Cytokine Research - ISSN 1079-9907 Roč. 22, - (2002), s. 741-749
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	Mouse MHC Class I Gene, Intragenic Downstream Regulatory Element
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LN00A079 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z5052915 - UMG-J
Annotation	The intragenic downstream regulatory element of the H2-K(b) gene (H2DRE) retains full competence to be induced by tumor necrosis factor-alpha (TNF-alpha) treatment. Chloramphenicol acetyltransferase (CAT) reporter gene assay revealed that NF-IL6 is able to elevate transcription from H2DRE. Moreover, transient transfection of an NF-IL6 expression vector increased both constitutive and TNF-alpha-induced mRNA levels of endogenous H2 class I genes, and transfection of an NF-IL6 dominant negative construct decreased the expression of endogenous H2 class I genes in a dose-dependent manner. Two potential NF-IL6 (C/EBPbeta) binding motifs within the H2DRE were uncovered. Using the EMSA and antibody supershift assay, we were able to confirm these two putative NF-IL6 binding motifs as a high-affinity and a low-affinity binding site. We conclude that the H2-K(b) gene belongs to target genes of the NF-IL6 (C/EBPbeta) in the course of the cellular response to TNF-alpha
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2005

Number of the records: 1