Mitochondrial histone-like DNA-binding proteins are essential for normal cell growth and mitochondrial function in Crithidia fasciculata
1.
SYSNO ASEP
0104423
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Mitochondrial histone-like DNA-binding proteins are essential for normal cell growth and mitochondrial function in Crithidia fasciculata
Title
Mitochondriální DNA vazebné proteiny podobné histonů jsou esenciální pro růst buněk a pro správnou funkci mitochondrie u Crithidia fasciculata
Author(s)
Avliyakulov, N. K. (US) Lukeš, Julius (PAU-O) Ray, D. S. (US)
Source Title
Eukaryotic Cell
- ISSN 1535-9778
Roč. 3, č. 2 (2004), s. 518-526
Number of pages
9 s.
Language
eng - English
Country
US - United States
Keywords
cell growth ; mitochondrial function ; Kinetoplastida
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
IAA5022302 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ
AV0Z6022909 - PAU-O, BC-A
Annotation
The Crithidia fasciculata KAP2 and KAP3 proteins are closely related kinetoplast-specific histone-like DNA-binding proteins. The KAP2 and KAP3 genes are 46% identical and are arranged in tandem on the chromosomal DNA. Disruption of both alleles of either gene alone shows no detectable phenotype. However, replacement of both copies of the sequence encoding the entire KAP2 and KAP3 locus increases maxicircle mRNA levels two- to fourfold. These double-knockout cells are viable but grow extremely slowly, have reduced respiration and very abnormal cell morphologies, and accumulate numerous large vacuoles. The extreme phenotype of these mutant cells suggests an important role for the KAP2 and KAP3 proteins in mitochondrial metabolism and cell growth
