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Monitoring toxicity, DNA damage, and somatic mutations in tobacco plants growing in soil heavily polluted with polychlorinated biphenyls

SYSNO ASEP	0099652
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Monitoring toxicity, DNA damage, and somatic mutations in tobacco plants growing in soil heavily polluted with polychlorinated biphenyls
Title	Monitorování toxicity, poškození DNA a somatické mutace u rostlin tabáku rostoucí na půdě s vysokým obsahem polychlorovaných bifenylů
Author(s)	Gichner, Tomáš (UEB-Q) Lovecká, P. (CZ) Kochánková, L. (CZ) Macková, M. (CZ) Demnerová, K. (CZ)
Source Title	Mutation Research - Genetic Toxicology and Environmental Mutagenesis. - : Elsevier - ISSN 1383-5718 Roč. 629, č. 1 (2007), s. 1-6
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	comet assay ; single-cell gel electrophoresis ; gas chromatography
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA521/05/0500 GA ČR - Czech Science Foundation (CSF)
Next source	Other public resourcesOther public resourcesOther public resources
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
DOI	10.1016/j.mrgentox.2006.11.013
Annotation	Heterozygous tobacco (Nicotiana tabacum var. xanthi) plants were cultivated in soil from a dump site highly polluted with polychlorinated biphenyls (PCBs) at Lhenice in South Bohemia, Czech Republic. The total amount of PCBs in the polluted soil, measured by gas chromatography varied from 165 to 265 mg kg(-1) of soil. In tobacco plants cultivated for 8 weeks in the polluted soil the amount of PCBs in the leaves varied from 11 to 28 and in the roots from 104 to 308 mg kg(-1) dry mass. The average leaf area of tobacco plants growing in the PCB-polluted soil was significantly reduced and the DNA damage in leaf nuclei, measured by the comet assay, was slightly but significantly increased compared with controls. The tobacco plants with increased DNA damage showed reduced growth and had distorted leaves. No increase in the frequency of somatic mutations was detected in tobacco plants growing in the PCB-polluted soil.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2008

Number of the records: 1