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Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli
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SYSNO ASEP 0098694 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli Title Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli Author(s) Chatchen, S. (TH)
Juříček, Miloslav (UEB-Q) RID, ORCID
Rueda, P. (ES)
Kertbundit, Sunee (UEB-Q)Source Title Journal of Biochemistry and Molecular Biology - ISSN 1225-8687
Roč. 39, č. 1 (2006), s. 16-21Number of pages 6 s. Language eng - English Country KR - Korea, Republic of Keywords antigen presentation ; canine parvo virus ; epitope ; papaya ringspot virus Subject RIV EF - Botanics Next source Other public resources CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation The coat protein (CP) of Papaya ringspot virus (PRSV) was analyzed for presentation of the antigenic peptide of animal virus, Canine parvovirus (CPV), in Escherichia coli (E. coli). The 45 nucleotides fragment coding for the 15-aa peptide epitope of the CPV-VP2 protein was either inserted into the PRSV-cp gene at the 5', 31 ends, both 5' and 3' ends or substituted into the 3' end of the PRSV cp gene. Each of the chimeric PRSV cp genes was cloned into the pRSET B vector under the control of the T7 promoter and transformed into E. coli. The recombinant coat proteins expressed from different chimeric PRSV-cp genes were purified and intraperitoneally injected into mice. All of the recombinant coat proteins showed strong immunogenicity and stimulate mice immune response. The recombinant coat proteins containing the CPV epitope insertion at the C terminus and at both N and C termini elicited ten times higher specific antisera in immunized mice compared with the other two recombinant coat proteins which contain the CPV epitope insertion at the N terminus and substitution at the C terminus. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2008
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