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Susceptibility of germ-free pigs to challenge with protease mutants of Salmonella enterica serovar Typhimurium
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SYSNO ASEP 0096910 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Susceptibility of germ-free pigs to challenge with protease mutants of Salmonella enterica serovar Typhimurium Title Citlivost bezmikrobních prasat ke stimulaci proteázovými mutanty Salmonella enterica serovar Typhimurium Author(s) Šplíchal, Igor (MBU-M) RID
Rychlík, I. (CZ)
Gregorová, D. (CZ)
Šebková, A. (CZ)
Trebichavský, Ilja (MBU-M)
Šplíchalová, Alla (MBU-M) RID, ORCID
Muneta, Y. (JP)
Mori, Y. (JP)Source Title Immunobiology. - : Elsevier - ISSN 0171-2985
Roč. 212, - (2007), s. 577-582Number of pages 6 s. Language eng - English Country DE - Germany Keywords salmonella ; protease mutants ; pig Subject RIV EE - Microbiology, Virology R&D Projects GA524/05/2248 GA ČR - Czech Science Foundation (CSF) Next source Other public resources CEZ AV0Z50200510 - MBU-M (2005-2011) Annotation Salmonella protease mutants, clpP and especially htrA, are candidate live oral vaccines in humans. We test the cytokine response of highly susceptible germ-free pigs to infection with Salmonella Typhimurium clpP and htrA mutants. Cytokine levels (IL-4, IL-10, IL-18 and IFN-gamma) were measured by ELISA in plasma and washes from the terminal small bowel 24 h after oral challenge. Unlike the infection with the wild type strain, no IFN-gamma response and low IL-18 intestinal levels were found in pigs infected with the protease mutants. Our results indicate that htrA and clpP attenuations should be used with caution in populations in which an increased number of immunocompromised individuals can be expected Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2008
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